human vegf r2 Search Results


vegfr2 expression  (MedChemExpress)
92
MedChemExpress vegfr2 expression
Effect of RBP4 on sprouting in vivo and in vitro. HUVECs were treated with 10 ng/ml RBP4 for 48 h, with or without 1 μM Ki8751 pretreatment for 12 h. A Relative mRNA expression of ANGPT2, TIE1, HEY1 and DLL4 in RBP4-treated HUVECs ( n = 3). B Relative mRNA expression of ANGPT2, TIE1, HEY1 and DLL4 in the control, RBP4 and RBP4 + Ki8751 groups ( n = 3). C , D Protein levels of <t>VEGFR2</t> in the three groups ( n = 3). E Immunofluorescence staining of DAPI (blue) and VEGFR2 (red) in HUVECs. Scale bar: 50 μm. F Co-IP examination of VEGFR2-RBP4 interaction. H , J , K Retinal IB4 staining of P14 in OIR and relative number of tip cells as well as filopodia. Scale bar: 200 μm ( n = 3). G , I Choroidal sprouting analysis in three groups ( n = 3). Scale bar: 500 μm. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001
Vegfr2 Expression, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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recombinant human vegfr2/kdr fc chimera protein, cf  (Bio-Techne corporation)
92
Bio-Techne corporation recombinant human vegfr2/kdr fc chimera protein, cf
Effect of RBP4 on sprouting in vivo and in vitro. HUVECs were treated with 10 ng/ml RBP4 for 48 h, with or without 1 μM Ki8751 pretreatment for 12 h. A Relative mRNA expression of ANGPT2, TIE1, HEY1 and DLL4 in RBP4-treated HUVECs ( n = 3). B Relative mRNA expression of ANGPT2, TIE1, HEY1 and DLL4 in the control, RBP4 and RBP4 + Ki8751 groups ( n = 3). C , D Protein levels of <t>VEGFR2</t> in the three groups ( n = 3). E Immunofluorescence staining of DAPI (blue) and VEGFR2 (red) in HUVECs. Scale bar: 50 μm. F Co-IP examination of VEGFR2-RBP4 interaction. H , J , K Retinal IB4 staining of P14 in OIR and relative number of tip cells as well as filopodia. Scale bar: 200 μm ( n = 3). G , I Choroidal sprouting analysis in three groups ( n = 3). Scale bar: 500 μm. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001
Recombinant Human Vegfr2/Kdr Fc Chimera Protein, Cf, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vegfr2  (Boster Bio)
94
Boster Bio vegfr2
Effect of RBP4 on sprouting in vivo and in vitro. HUVECs were treated with 10 ng/ml RBP4 for 48 h, with or without 1 μM Ki8751 pretreatment for 12 h. A Relative mRNA expression of ANGPT2, TIE1, HEY1 and DLL4 in RBP4-treated HUVECs ( n = 3). B Relative mRNA expression of ANGPT2, TIE1, HEY1 and DLL4 in the control, RBP4 and RBP4 + Ki8751 groups ( n = 3). C , D Protein levels of <t>VEGFR2</t> in the three groups ( n = 3). E Immunofluorescence staining of DAPI (blue) and VEGFR2 (red) in HUVECs. Scale bar: 50 μm. F Co-IP examination of VEGFR2-RBP4 interaction. H , J , K Retinal IB4 staining of P14 in OIR and relative number of tip cells as well as filopodia. Scale bar: 200 μm ( n = 3). G , I Choroidal sprouting analysis in three groups ( n = 3). Scale bar: 500 μm. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001
Vegfr2, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human elisa kit  (BioVendor Instruments)
93
BioVendor Instruments human elisa kit
Effect of RBP4 on sprouting in vivo and in vitro. HUVECs were treated with 10 ng/ml RBP4 for 48 h, with or without 1 μM Ki8751 pretreatment for 12 h. A Relative mRNA expression of ANGPT2, TIE1, HEY1 and DLL4 in RBP4-treated HUVECs ( n = 3). B Relative mRNA expression of ANGPT2, TIE1, HEY1 and DLL4 in the control, RBP4 and RBP4 + Ki8751 groups ( n = 3). C , D Protein levels of <t>VEGFR2</t> in the three groups ( n = 3). E Immunofluorescence staining of DAPI (blue) and VEGFR2 (red) in HUVECs. Scale bar: 50 μm. F Co-IP examination of VEGFR2-RBP4 interaction. H , J , K Retinal IB4 staining of P14 in OIR and relative number of tip cells as well as filopodia. Scale bar: 200 μm ( n = 3). G , I Choroidal sprouting analysis in three groups ( n = 3). Scale bar: 500 μm. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001
Human Elisa Kit, supplied by BioVendor Instruments, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit anti-vegfr2 (flk-1,kdr) antibody  (Zytomed Inc)
90
Zytomed Inc rabbit anti-vegfr2 (flk-1,kdr) antibody
Effect of RBP4 on sprouting in vivo and in vitro. HUVECs were treated with 10 ng/ml RBP4 for 48 h, with or without 1 μM Ki8751 pretreatment for 12 h. A Relative mRNA expression of ANGPT2, TIE1, HEY1 and DLL4 in RBP4-treated HUVECs ( n = 3). B Relative mRNA expression of ANGPT2, TIE1, HEY1 and DLL4 in the control, RBP4 and RBP4 + Ki8751 groups ( n = 3). C , D Protein levels of <t>VEGFR2</t> in the three groups ( n = 3). E Immunofluorescence staining of DAPI (blue) and VEGFR2 (red) in HUVECs. Scale bar: 50 μm. F Co-IP examination of VEGFR2-RBP4 interaction. H , J , K Retinal IB4 staining of P14 in OIR and relative number of tip cells as well as filopodia. Scale bar: 200 μm ( n = 3). G , I Choroidal sprouting analysis in three groups ( n = 3). Scale bar: 500 μm. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001
Rabbit Anti Vegfr2 (Flk 1,Kdr) Antibody, supplied by Zytomed Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human vegfr2/kdr/flk-1 apc-conjugated antibody  (Bio-Techne corporation)
94
Bio-Techne corporation human vegfr2/kdr/flk-1 apc-conjugated antibody
Effect of RBP4 on sprouting in vivo and in vitro. HUVECs were treated with 10 ng/ml RBP4 for 48 h, with or without 1 μM Ki8751 pretreatment for 12 h. A Relative mRNA expression of ANGPT2, TIE1, HEY1 and DLL4 in RBP4-treated HUVECs ( n = 3). B Relative mRNA expression of ANGPT2, TIE1, HEY1 and DLL4 in the control, RBP4 and RBP4 + Ki8751 groups ( n = 3). C , D Protein levels of <t>VEGFR2</t> in the three groups ( n = 3). E Immunofluorescence staining of DAPI (blue) and VEGFR2 (red) in HUVECs. Scale bar: 50 μm. F Co-IP examination of VEGFR2-RBP4 interaction. H , J , K Retinal IB4 staining of P14 in OIR and relative number of tip cells as well as filopodia. Scale bar: 200 μm ( n = 3). G , I Choroidal sprouting analysis in three groups ( n = 3). Scale bar: 500 μm. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001
Human Vegfr2/Kdr/Flk 1 Apc Conjugated Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human vegf r2 (kdr)/fc chimera  (ReLIA Diagnostics)
90
ReLIA Diagnostics human vegf r2 (kdr)/fc chimera
Effect of RBP4 on sprouting in vivo and in vitro. HUVECs were treated with 10 ng/ml RBP4 for 48 h, with or without 1 μM Ki8751 pretreatment for 12 h. A Relative mRNA expression of ANGPT2, TIE1, HEY1 and DLL4 in RBP4-treated HUVECs ( n = 3). B Relative mRNA expression of ANGPT2, TIE1, HEY1 and DLL4 in the control, RBP4 and RBP4 + Ki8751 groups ( n = 3). C , D Protein levels of <t>VEGFR2</t> in the three groups ( n = 3). E Immunofluorescence staining of DAPI (blue) and VEGFR2 (red) in HUVECs. Scale bar: 50 μm. F Co-IP examination of VEGFR2-RBP4 interaction. H , J , K Retinal IB4 staining of P14 in OIR and relative number of tip cells as well as filopodia. Scale bar: 200 μm ( n = 3). G , I Choroidal sprouting analysis in three groups ( n = 3). Scale bar: 500 μm. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001
Human Vegf R2 (Kdr)/Fc Chimera, supplied by ReLIA Diagnostics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Effect of RBP4 on sprouting in vivo and in vitro. HUVECs were treated with 10 ng/ml RBP4 for 48 h, with or without 1 μM Ki8751 pretreatment for 12 h. A Relative mRNA expression of ANGPT2, TIE1, HEY1 and DLL4 in RBP4-treated HUVECs ( n = 3). B Relative mRNA expression of ANGPT2, TIE1, HEY1 and DLL4 in the control, RBP4 and RBP4 + Ki8751 groups ( n = 3). C , D Protein levels of VEGFR2 in the three groups ( n = 3). E Immunofluorescence staining of DAPI (blue) and VEGFR2 (red) in HUVECs. Scale bar: 50 μm. F Co-IP examination of VEGFR2-RBP4 interaction. H , J , K Retinal IB4 staining of P14 in OIR and relative number of tip cells as well as filopodia. Scale bar: 200 μm ( n = 3). G , I Choroidal sprouting analysis in three groups ( n = 3). Scale bar: 500 μm. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001

Journal: Journal of Neuroinflammation

Article Title: Succinate-induced macrophage polarization and RBP4 secretion promote vascular sprouting in ocular neovascularization

doi: 10.1186/s12974-023-02998-1

Figure Lengend Snippet: Effect of RBP4 on sprouting in vivo and in vitro. HUVECs were treated with 10 ng/ml RBP4 for 48 h, with or without 1 μM Ki8751 pretreatment for 12 h. A Relative mRNA expression of ANGPT2, TIE1, HEY1 and DLL4 in RBP4-treated HUVECs ( n = 3). B Relative mRNA expression of ANGPT2, TIE1, HEY1 and DLL4 in the control, RBP4 and RBP4 + Ki8751 groups ( n = 3). C , D Protein levels of VEGFR2 in the three groups ( n = 3). E Immunofluorescence staining of DAPI (blue) and VEGFR2 (red) in HUVECs. Scale bar: 50 μm. F Co-IP examination of VEGFR2-RBP4 interaction. H , J , K Retinal IB4 staining of P14 in OIR and relative number of tip cells as well as filopodia. Scale bar: 200 μm ( n = 3). G , I Choroidal sprouting analysis in three groups ( n = 3). Scale bar: 500 μm. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001

Article Snippet: Administration of the VEGFR2 inhibitor Ki8751 (1 μM for 12 h, #HY-12038, MCE) reduced VEGFR2 expression and attenuated RBP4 promotion of ANGPT2 and TIE1 as well as inhibition of HEY1 and DLL4 (Fig. B).

Techniques: In Vivo, In Vitro, Expressing, Control, Immunofluorescence, Staining, Co-Immunoprecipitation Assay